Analysis Methods for Amino Acids

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Method Code Method Reference Analytes Method Type
AA0001 AOAC Official Method 982.30 GLYCINE,LEUCINE,PHENYLALANINE,ARGININE,TRYPTOPHAN,TYROSINE,GLUTAMIC ACID,ISOLEUCINE,VALINE,ALANINE,HISTIDINE,LYSINE,ASPARTIC ACID,SERINE,PROLINE,CYSTINE/CYSTEINE,THREONINE,METHIONINE Amino Acid Analyzer
AA0002 AOAC Official Method 988.15 TRYPTOPHAN Alkaline hydrolysis
AA0003 AOAC Official Method 994.12 CYSTINE/CYSTEINE, METHIONINE Acid hydrolysis
AA0004 Journal of Chromatography A 670:59-66 (1994) GLYCINE,LEUCINE,PHENYLALANINE,ARGININE,TRYPTOPHAN,TYROSINE,GLUTAMIC ACID,ISOLEUCINE,VALINE,ALANINE,HISTIDINE,LYSINE,ASPARTIC ACID,SERINE,PROLINE,CYSTINE/CYSTEINE,THREONINE,METHIONINE Precolumn deriv and HPLC (UV)
AA0005 Journal of Micronutrient Analysis 7:27-35 (1990) TRYPTOPHAN Reverse phase HPLC (UV)
AA0006 Journal of Chromatography, 431:271-284 (1988)/Agilent Publication 5980-1193 (2000)/ Agilent Application Note 5990-4547(2010)/ Analytical Biochemistry, 177:318-322 (1989) GLYCINE,LEUCINE,PHENYLALANINE,ARGININE,TYROSINE,GLUTAMIC ACID,VALINE,ISOLEUCINE,ALANINE,HISTIDINE,LYSINE,ASPARTIC ACID,SERINE,PROLINE,THREONINE,CYSTINE/CYSTEINE,METHIONINE Hydrolysis, derivatization, Reverse phase HPLC
AA0007 AOAC Official Method 988.15/Journal of Chromatography, 431:271-284 (1988)/Agilent Publication 5980-1193 (2000)/ Agilent Application Note 5990-4547(2010) TRYPTOPHAN Hydrolysis, derivatization, HPLC-FL
AA0008 AOAC Official Method 994.12/Journal of Chromatography A 670:59-66 (1994) CYSTINE/CYSTEINE, METHIONINE Oxidation, hydrolyzed, pre-column derivatization, HPLC-UV
AA0009 Official Journal of the European Union: Commission Regulation 152 (2009), Annex III, Part F. GLYCINE,LEUCINE,PHENYLALANINE,ARGININE,TYROSINE,GLUTAMIC ACID,ISOLEUCINE,VALINE,ALANINE,HISTIDINE,LYSINE,ASPARTIC ACID,PROLINE,SERINE,CYSTINE/CYSTEINE,THREONINE,METHIONINE Oxidation, hydrolyzed, Ion exchange, photometric detection (Amino Acid analyzer)
AA0010 Official Journal of the European Union: Commission Regulation 152 (2009), Annex III, Part G/EN ISO 13904 TRYPTOPHAN HPLC (fluorescence)
AA0011 Commission Directive 98/64/EC GLYCINE,LEUCINE,PHENYLALANINE,ARGININE,GLUTAMIC ACID,ISOLEUCINE,VALINE,ALANINE,HISTIDINE,LYSINE,ASPARTIC ACID,PROLINE,SERINE,CYSTINE/CYSTEINE,THREONINE,METHIONINE Oxidation, hydrolyzed, Ion exchange, photometric detection (Amino Acid analyzer)
AA0012 Journal of Chromatography, 431:271-284 (1988)/Agilent Publication 5980-1193 (2000)/ Agilent Application Note 5990-4547(2010) FREE ARGININE,FREE HISTIDINE,FREE LEUCINE,FREE TYROSINE,FREE THREONINE,FREE ASPARTIC ACID,FREE SERINE,FREE GLUTAMINE,FREE TRYPTOPHAN,FREE CYSTINE,FREE GLUTAMIC ACID,FREE PROLINE,FREE ALANINE,FREE PHENYLALANINE,FREE VALINE,FREE ISOLEUCINE,FREE LYSINE,FREE GLYCINE,FREE METHIONINE,FREE ASPARAGINE Extraction, derivatization, Reverse phase HPLC
AA0013 Republic of Korea, Ministry of Food and Drug Safety, MFDS Food Code (Notification No. 2019-31, 2019.4.26) Chapter 8, 2.1.3.3 GLYCINE,LEUCINE,PHENYLALANINE,ARGININE,TRYPTOPHAN,TYROSINE,GLUTAMIC ACID,ISOLEUCINE,VALINE,ALANINE,HISTIDINE,LYSINE,ASPARTIC ACID,SERINE,PROLINE,CYSTINE/CYSTEINE,THREONINE,METHIONINE Amino Acid Analyzer
AA0014 Journal of Chromatography A 670:59-66 (1994)/Waters Method, Analysis of Amino Acids in Feeds and Foods Using a Modification of the ACCQ Tag Method™ for Amino Acid Analysis 1995; Rev. 1.1. ALANINE, ARGININE, ASPARTIC ACID, GLUTAMIC ACID, GLYCINE, HISTIDINE, ISOLEUCINE, LEUCINE, LYSINE, PHENYLALANINE, PROLINE, SERINE, THREONINE, TYROSINE, VALINE Reverse-phase UPLC with UV Detection
AA0015 AOAC Official Method 994.12/Journal of Chromatography A 670:59-66 (1994)/Waters Method, Analysis of Amino Acids in Feeds and Foods Using a Modification of the ACCQ Tag Method™ for Amino Acid Analysis 1995; Rev. 1.1. CYSTINE/CYSTEINE, METHIONINE Reverse-phase UPLC with UV Detection