Analysis Methods for Bio-Actives

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Method Code Method Reference Analytes Method Type
BA0001 AACC Method 80-04 RAFFINOSE,STACHYOSE HPLC-RI
BA0002 Analytical Biochemistry 77:536-539 (1977) PHYTIC ACID Acid extraction, ferric iron precipitation, acid hydrolysis, anion exchange resin column. 
BA0003 AOAC Official Method 986.11 PHYTIC ACID Anion Exchange/Colorimetric (Phosphorus)
BA0004 AOCS Official Method Ba 12-75/AACC 22-40/71-10 TRYPSIN INHIBITOR Spectrophotometric
BA0005 AOCS Official Method Ba 7-58 FREE GOSSYPOL Spectrophotometric
BA0006 AOCS Official Method Ba 8-78 TOTAL GOSSYPOL Spectrophotometric
BA0007 Cereal Chemistry 66:510-515 (1989)/Journal of Agricultural and Food Chemistry 42:2726-2731 (1994) PHYTIC ACID Acid extraction, anion exchange, HPLC RI
BA0008 Sugar.01 Corn Refiners Association E-29 (1978) RAFFINOSE GC-FID
BA0009 Journal of Agricultural and Food Chemistry 19:551-554 (1971) GLUCOSE,FRUCTOSE,RAFFINOSE,STACHYOSE,SUCROSE,MALTOSE GLC
BA0010 Journal of Agricultural and Food Chemistry 32:530-533 (1984)/Journal of the Association of Official Analytical Chemists, 67(3):503-506 (1984). TOTAL GLYCITEIN,TOTAL GENISTEIN,TOTAL DAIDZEIN Ethanol extraction, hydrolysis, Sep-PAK, Reverse-phase HPLC
BA0011 Journal of Agricultural and Food Chemistry 47:2697-2704 (1999) TOTAL ISOFLAVONES,TOTAL GLYCITEIN,TOTAL GENISTEIN,TOTAL DAIDZEIN HPLC
BA0012 Lipids 22:667-668 (1987) LECTINS Agglutination/Gel electrophoresis
BA0013 Boehringer Mannheim R Biopharm cat# 428167 (1997) RAFFINOSE UV spec (test kit)
BA0014 AACC Method 80-04/Journal of Agricultural and Food Chemistry 44:1470-1474 (1996) RAFFINOSE,STACHYOSE,SUCROSE HPLC-RI
BA0015 Journal of Agricultural and Food Chemistry 42:1905-1913 (1994) COUMESTROL,SINAPINE Acid hydrolysis/Reverse phase HPLC (Diode array)
BA0016 Archives of Biochemistry and Biophysics, 54:223-231 (1955) LECTINS Hemagglutinativy activity/Photometric/Rabbit Red Blood Cells
BA0017 ISO Method 9167-1 GLUCOIBERIN,GLUCONAPIN,PROGOITRIN,TOTAL ALKYL GLUCOSINOLATES,4-HYDROXYGLUCOBRASSICIN,GLUCORAPHANIN,TOTAL INDOLYL GLUCOSINOLATES,TOTAL GLUCOSINOLATES,GLUCONAPOLEIFERIN,4-METHOXYGLUCOBRASSICIN,NEOGLUCOBRASSICIN,GLUCOBRASSICIN,GLUCONASTURTIIN,GLUCOBRASSICANAPIN,GLUCOALYSSIN,EPI-PROGOITRIN Reverse phase HPLC
BA0018 Journal of Agricultural and Food Chemistry 42:1317-1322 (1994)/Journal of Agricultural and Food Chemistry 46:575-579 (1998)/Journal of Agricultural and Food Chemistry 50:1368-1373 (2002) SINAPINE Ion-exchange purification with UV spectrophotometric detection (IE-UV)/Reversed-Phase Chromatography/HPLC
BA0019 Phytochemistry 25:223-230 (1986)/ Journal of Agricultural and Food Chemistry 41:1256-1260 (1993) / Journal of Agricultural and Food Chemistry 42:2196-2200 (1994) / Journal of the Science of Food and Agriculture 78:405-416 (1998) / Journal of Agricultural and Food Chemistry 48:1758-1762 (2000) / Journal of the Science of Food and Agriculture 80:1094-1117 (2000) CONDENSED TANNINS (SOLUBLE),TOTAL TANNINS,CONDENSED TANNINS (INSOLUBLE) Spectrophotometric, Colorimetric, Proanthocyanidin assay, butanol/HCl extraction
BA0020 AOAC Official Method 2001.10 TOTAL ISOFLAVONES,TOTAL GLYCITEIN,TOTAL GENISTEIN,TOTAL DAIDZEIN Extraction/Liquid Chromatography
BA0021 AOCS Official Method Ba 12-75/Cereal Chemistry 51:376-384 (1974) TRYPSIN INHIBITOR Spectrophotometric
BA0023 Archives of Biochemistry and Biophysics, 54:223-231 (1955)/Analytical Biochemistry, 68, 651-653 (1975)/Journal of Agricultural and Food Chemistry, 20: 87-90, (1972) LECTINS Hemagglutinating activity/Photometric/Rabbit Red Blood Cells
BA0024 Advances in Experimental Medicine and Biology 505: 61-76 (2002)/Journal of Chromatography B 777: 129-138 (2002) TOTAL GLYCITEIN,TOTAL GENISTEIN,TOTAL DAIDZEIN Extraction/Liquid Chromatography, Diode Array detection
BA0025 Journal of Agricultural and Food Chemistry 19:551-554 (1971)/Brobst, Methods in Carbohydrate Chemistry, Vol 6 (1972) RAFFINOSE,STACHYOSE GC-FID
BA0026 EN 12014-5/12014-3 NITRATE (NO3),NITRITE (NO2) Enzymatic reduction/Spectrometric detection
BA0027 Journal of AOAC International 80:549-554 (1997) TOTAL GLYCOALKALOIDS,CHACONINE,SOLANINE SPE, Reversed phase HPLC (UV)
BA0028 AOCS Official Method Ch 6-91 / Journal of Chromatography A 935:173-201 (2001) / Proceedings of the Estonian Academy of Sciences, Chemistry 56:59-66 (2007) TOTAL STEROLS,STIGMASTEROL,BETA-SITOSTEROL,CHOLESTEROL,CAMPESTEROL,BRASSICASTEROL TLC and GC-FID
BA0029 Food Chemistry, 113:1218-1225 (2009) LECTIN_SOYBEAN,LECTINS ELISA
BA0030 AOAC Official Method 941.15/2005.07 LUTEIN,ZEAXANTHIN Spectrophotometry/Liquid Chromatography
BA0031 AOAC Official Method 2001.10/AACC Method 20-20 / Journal of Agricultural and Food Chemistry 52: 2647-2651 (2004) TOTAL GLYCITEIN,TOTAL GENISTEIN,TOTAL DAIDZEIN Extraction/Liquid Chromatography
BA0034 Journal of the American Oil Chemists' Society 57: 143-144 (1980) STACHYOSE HPLC
BA0035 Journal of Agricultural and Food Chemistry 51:4532-4538 (2003)/Food Science Biotechnology 15:91-95 (2006)/Peanut Science 13:4-7 (1986) LECTINS NaCl extraction, Affinity chromatography, Hemagglutinating activity/Photometric/Rabbit Red Blood Cells
BA0036 Journal of the Science of Food and Agriculture 58:321-329 (1992) CONDENSED TANNINS Colorimetric
BA0037 American Journal of Enology and Viticulture, 28(1): 49-55 (1977) TOTAL PHENOLICS Folin-Ciocalteu Method
BA0038 Phytochemistry 25:223-230 (1986)/ Journal of Agricultural and Food Chemistry 48:1758-1762 (2000) CONDENSED TANNINS (SOLUBLE),TOTAL TANNINS,CONDENSED TANNINS (INSOLUBLE) Acid/Butanol auto-oxidation/Vis spectrophotometry
BA0039 Journal of the American Oil Chemists' Society 78:903-910 (2001) /Journal of the American Oil Chemists' Society 87:147-155 (2010) SINAPINE Extraction, HPLC-UV
BA0040 AOAC Official Method 2001.10/Journal of Liquid Chromatography & Related Technologies, 27:315-324 (2004) TOTAL GLYCITEIN,TOTAL GENISTEIN,TOTAL DAIDZEIN Extraction/Liquid Chromatography
BA0041 Journal of Agricultural and Food Chemistry 28:1313-1315 (1980) PHYTIC ACID Acid extraction, ferric iron precipitation, acid hydrolysis, anion exchange resin column, Wade reagent. 
BA0042 Journal of AOAC INTERNATIONAL 89:1443-1446 (2006)/Journal of Agricultural and Food Chemistry 53:1914-1920 (2005)/Biomedical Chromatography 21:755-761 (2007) OLEANOLIC ACID Hydrolyzed, HPLC-UV
BA0043 Journal of the American Oil Chemists' Society 92:1085-1092 (2015) LECTINS ELISA
BA0045 AOCS Method Ba 12-75/Cereal Chemistry 51:376-384 (1974)/Cereal Chemistry 58:42-45 (1981)/Cereal Chemistry 66:415-422 (1989) TRYPSIN INHIBITOR Spectrophotometric - high throughput
BA0046 Essers, S.A.J.A., Bosveld, M., Van Grift, R.M.D. and Voragen, A.G.J. (1993), Studies on the quantification of specific cyanogens in cassava products and introduction of a new chromogen. J. Sci. Food Agric., 63: 287–296. HYDROCYANIC ACID Acid extraction, enzymatic hydrolysis, conversion to HCN, Konig reaction, spectrophotometric