Analysis Methods for Vitamins

< Back to Analysis Methods

Method Code Method Reference Analytes Method Type
VT0001 AACC Official Method 86-31 VITAMIN B6 Microbiological (B6 activity)
VT0002 AACC Official Method 86-47 VITAMIN B9 Microbiological/enzymatic (total folate)
VT0003 AACC Official Method 86-51 VITAMIN B3 Microbiological
VT0004 AACC Official Method 86-70 VITAMIN B2 Fluorometric
VT0005 AACC Official Method 86-72 VITAMIN B2 Microbiological/Titration
VT0006 AACC Official Method 86-80 VITAMIN B1, VITAMIN B2 Fluorometric
VT0007 AOAC Official Method 940.33/960.46 VITAMIN B2 Turbidimetric
VT0008 AOAC Official Method 941.15 BETA-CAROTENE Spectrophotometry
VT0009 AOAC Official Method 942.23 VITAMIN B1 Fluorometric
VT0010 AOAC Official Method 942.23/953.17/957.17 VITAMIN B1 Fluorometric
VT0011 AOAC Official Method 944.13/960.46 VITAMIN B3 Turbidimetric
VT0012 AOAC Official Method 960.46/992.05 VITAMIN B9 Microbioassay/Turbidimetric
VT0013 AOAC Official Method 961.15 VITAMIN B6 Microbioassay
VT0014 AOAC Official Method 970.65 VITAMIN B2 Fluorometric
VT0015 AOAC Official Method 971.30 BETA-TOCOPHEROL, ALPHA-TOCOPHEROL (VITAMIN E), DELTA-TOCOPHEROL, GAMMA-TOCOPHEROL, TOTAL TOCOPHEROLS Colorimetry
VT0016 AOAC Official Method 971.30 with HPLC Quantitation BETA-TOCOPHEROL, ALPHA-TOCOPHEROL (VITAMIN E), DELTA-TOCOPHEROL, GAMMA-TOCOPHEROL, TOTAL TOCOPHEROLS Extract/Saponify/HPLC
VT0017 AOAC Official Method 992.05 VITAMIN B9 Microbioassay
VT0018 Journal of Agricultural and Food Chemistry 31:1330-1333 (1983) BETA-TOCOPHEROL, ALPHA-TOCOPHEROL (VITAMIN E), DELTA-TOCOPHEROL, GAMMA-TOCOPHEROL, TOTAL TOCOPHEROLS HPLC
VT0019 Journal of the AOCS 61:1231-1234 (1984) BETA-TOCOPHEROL, ALPHA-TOCOPHEROL (VITAMIN E), DELTA-TOCOPHEROL, GAMMA-TOCOPHEROL, TOTAL TOCOPHEROLS HPLC (Fluorescence)
VT0020 Supelco Chromatography Products Guide p. 543 (2001) VITAMIN B1, VITAMIN B2 HPLC (Fluorescence)
VT0021 Journal of AOAC International, 85 (3):682-691(2002)/European Food Research and Technology, 235:873-879 (2012) VITAMIN K1 Lipase hydrolysis, extraction, UPLC-MS/MS
VT0022 AOAC Official Method 945.74 VITAMIN B5 Turbidimetric
VT0023 AOAC Official Method 992.27/999.15 VITAMIN K1 HPLC
VT0024 AOAC Official Method 2005.07/Journal of Liquid Chromatography, 10: 643-653, (1987) ALPHA CAROTENE, BETA CAROTENE Saponification, extraction, HPLC-UV
VT0025 AOAC Official Method 992.05/960.46/Methods of Analysis for Infant Formulas, Infant Formula Council, Atlanta, Georgia, Section C-2 (1985). VITAMIN B9 Microbioassay/Turbidimetric
VT0026 Journal of Food Science, 50:121-124 (1985)/Journal of Agricultural and Food Chemistry, 31:1330-1333 (1983)/Journal of the Association of Official Analytical Chemists, 63:1258-1261 (1980) BETA-TOCOPHEROL, ALPHA-TOCOPHEROL (VITAMIN E), DELTA-TOCOPHEROL, GAMMA-TOCOPHEROL, TOTAL TOCOPHEROLS, ALPHA TOCOTRIENOL, BETA TOCOTRIENOL, GAMMA TOCOTRIENOL, DELTA TOCOTRIENOL Extracted with hot hexane HPLC (Fluorescence)
VT0027 AOAC Official Method 941.15/Journal of Liquid Chromatography, 10: 643-653, (1987) BETA-CAROTENE,CRYPTOXANTHIN Spectrophotometry
VT0028 European Standard No. BS EN 14122:2003, "Foodstuffs-Determination of Vitamin B1 by HPLC,"(2003). VITAMIN B1 HPLC
VT0029 AOAC Official Method 969.40 ALPHA-TOCOPHEROL (VITAMIN E) Extraction followed by GC analysis
VT0030 AOCS Official Method Ce 8-89 BETA-TOCOPHEROL, ALPHA-TOCOPHEROL (VITAMIN E), DELTA-TOCOPHEROL, GAMMA-TOCOPHEROL, TOTAL TOCOPHEROLS HPLC - dilute and shoot
VT0031 Journal of Biological Chemistry 156:643-652 (1944) CHOLINE Reineckate Method (Wet Chem/Spectrophotometric)
VT0032 Journal of AOAC International, 85:682-691 (2002)/ European Food Research and Technology, 235:873-879 (2012)/AOAC Official Method 999.15/USP24-NF19:2321-2322 (2000) VITAMIN K1 HPLC
VT0033 Journal of AOAC International, 85:682-691 (2002)/USP35-NF30: 1571-1572 (2012). VITAMIN K1 Saponification/HPLC (post column reduction/Fluorescence detection)
VT0034 AOAC Official Method 967.22 VITAMIN C Microfluorometry
VT0035 Brubacher, Methods for the Determination of Vitamins in Food, p. 97-106 (1985) BETA-TOCOPHEROL, ALPHA-TOCOPHEROL (VITAMIN E), DELTA-TOCOPHEROL, GAMMA-TOCOPHEROL, TOTAL TOCOPHEROLS Saponification/HPLC (Fluorescence)
VT0036 AOAC Official Method 942.23/Journal of Chromatography A, 83:455-460 (1973) VITAMIN B1 Fluorometric
VT0037 AOAC Official Method 970.65/Journal of Chromatography A, 83:455-460 (1973) VITAMIN B2 Fluorometric
VT0038 AOAC Official Method 961.14/Journal of AOAC International, 72:244-247 (1989) VITAMIN B3 Autoclave Extraction, Reverse phase HPLC
VT0039 AOAC Official Method 2004.05/Journal of Agricultural and Food Chemistry, 51:5315-5318 (2003) VITAMIN B9 Microbiological/enzymatic/turbidimetric (total folate)
VT0040 Journal of AOAC International,73: 805-808 (1990) VITAMIN B9 Microbiological/enzymatic
VT0041 AOAC Official Method 971.30/ Journal of Agricultural and Food Chemistry 31:1330-1333 (1983) BETA-TOCOPHEROL,ALPHA-TOCOPHEROL (VITAMIN E),GAMMA-TOCOTRIENOL,DELTA-TOCOPHEROL,BETA-TOCOTRIENOL,ALPHA-TOCOTRIENOL,DELTA-TOCOTRIENOL,GAMMA-TOCOPHEROL,TOTAL TOCOPHEROLS,TOTAL TOCOTRIENOLS Saponification/HPLC
VT0042 AOAC Official Method 971.30/Journal of Agricultural and Food Chemistry 51:3940-3944 (2003)/Journal of Chromatography A 1218:4709-4713 (2011) BETA-TOCOPHEROL,ALPHA-TOCOPHEROL (VITAMIN E),GAMMA-TOCOTRIENOL,BETA-TOCOTRIENOL,ALPHA-TOCOTRIENOL,DELTA-TOCOPHEROL,DELTA-TOCOTRIENOL,GAMMA-TOCOPHEROL,TOTAL TOCOTRIENOLS,TOTAL TOCOPHEROLS Saponification, Extraction, Normal Phase HPLC
VT0043 AOAC Official Method 999.15/Journal of Agricultural and Food Chemistry 48:6325-6331 VITAMIN K1 HPLC
VT0044 AOAC Official Method 999.15/Journal of Agricultural and Food Chemistry 48:6325-6331 (2000)/Journal of Agricultural and Food Chemistry 54:463-647 (2006) VITAMIN K1 HPLC
VT0045 AOAC Official Method 999.15/Journal of AOAC International, 85:682-691 (2002)/European Food Research and Technology, 235:873-879 (2012) VITAMIN K1 HPLC
VT0046 Journal of the American Oil Chemists' Society, 61:1231-1234 (1984)/Analytical Sciences, 21:1545-1548 (2005) BETA-TOCOPHEROL, ALPHA-TOCOPHEROL (VITAMIN E), DELTA-TOCOPHEROL, GAMMA-TOCOPHEROL, TOTAL TOCOPHEROLS, ALPHA TOCOTRIENOL, BETA TOCOTRIENOL, GAMMA TOCOTRIENOL, DELTA TOCOTRIENOL, TOTAL TOCOTRIENOLS HPLC with Fluorescence Detection
VT0047 AOAC Official Method 974.29/Journal of Agricultural and Food Chemistry 55:10744-10750 (2007) BETA-CAROTENE Colorimetry
VT0048 Journal of Chromatography A, 881(1-2):309-316 (2000) VITAMIN C Liquid chromatographic with DTT as precolumn reductant
VT0049 Journal of AOAC International 76:1156-1160 (1993) VITAMIN B1 (THIAMIN) Autoclave extraction, derivatization, HPLC FL
VT0050 Journal of Agricultural and Food Chemistry 49: 1450–1454 VITAMIN B2 (RIBOFLAVIN) Reverse phase HPLC FL
VT0051 Cereal Chemistry 88:397-399 VITAMIN B3 (NIACIN) Isotope Dilution GC-TOF-MS
VT0052 Republic of Korea, Ministry of Food and Drug Safety, MFDS Food Code (Notification No. 2019-31, 2019.4.26) Chapter 8, 2.2.2.15 VITAMIN B7 (BIOTIN) Extraction, Immunoaffinity column, LC-UV or LCMS
VT0053 Journal of Liquid Chromatography & Related Technologies 22:2925-2934 (1999) ALPHA TOCOPHEROL (VITAMIN E) Ethanol precipitation, saponification, hexane/toluene extraction, HPLC
VT0054 AOAC Official Method 941.15/Journal of AOAC International 87:1070-1082 (2004) BETA-CAROTENE Extraction with acetone or alcohol, Reverse phase HPLC
VT0055 Journal of AOAC International, 85:682-691 (2002)/ European Food Research and Technology, 235:873-879 (2012)/AOAC Official Method 999.15/USP24-NF19:2321-2322 (2000)/Journal of Agricultural and Food Chemistry 63:1076-1081 (2015) VITAMIN K1 Extraction with DMSO and hexane. Purified on SPE. HPLC-MS/MS, C30 Reverse Phase column. 
VT0056 Walker, P. G., Gordon, S.L., Brennan, R. M. and Hancock, R.D. (2006), A high-throughput monolithic HPLC method for rapid Vitamin C phenotyping of berry fruit. Phytochem. Anal., 17: 284–290 VITAMIN C Extraction with metaphosphoric acid. Monolithic C18 column with HPLC photodiode array detection.